Learning the principles of molecular biology analyses techniques, ability to perform these techniques in guidance with an assistant, ability to interpret experimental results, optimizing the experimental procedures according to the results obtained
Prerequisite(s)
-
Corequisite(s)
-
Special Requisite(s)
-
Instructor(s)
Assist. Prof. Dr. Pelin Özfiliz Kılbaş, Assist. Prof. Dr. Özge Rencüzoğulları, Assist. Prof. Dr. Burcu Ayhan Şahin
Course Assistant(s)
Araş. Gör. Pelin Özfiliz, Araş. Gör. Dr. Özge Berrak Rencüzogulları, Araş. Gör. Burcu Ayhan Şahin, Araş. Gör. Tamer Gümüş
1. Editörler Güler Temizkan ve Nazlı Arda, Moleküler Biyolojide Kullanılan Yöntemler, Nobel Tıp Kitapevi, İstanbul, 2007 (ISBN: 9789754205831)
2. Editörler Atilla Özalpan ve Narçin P. Ünsal, Genomik Uygulamalar, T.C. İstanbul Kültür Üniversitesi Yayınları, İstambul, 2008
3. Dale, von Schantz, From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, John Wiley & Sons, Ltd, USA, 2007 (ISBN: 9780470017340)
Other Sources
Course Schedules
Week
Contents
Learning Methods
1. Week
Security in molecular biology laboratory;
Learning the laboratory and commonly used laboratory equipment
Using micropipettes, Laboratory calculations;
Preparation of solutions and tampons to be used in laboratory;
pH adjustments
Oral presentation;
Laboratory
2. Week
Common precedures used fro isolation and purificaiton of macromolecules I;
Genomic DNA isolation from bacteria, Spectrophotometric methods;
Quantification of genomic DNA concentrations using spectrohotometry
Oral presentation;
Laboratory
3. Week
Common precedures used fro isolation and purificaiton of macromolecules II;
Genomic DNA isolation from plants, Spectrophotometric methods;
Quantification of genomic DNA concentrations using spectrohotometry
Oral presentation;
Laboratory
4. Week
Common precedures used fro isolation and purificaiton of macromolecules III;
Genomic DNA isolation from mammalian cells, Spectrophotometric methods;
Quantification of genomic DNA concentrations using spectrohotometry
Oral presentation;
Laboratory
5. Week
Common precedures used fro isolation and purificaiton of macromolecules III;
Genomic DNA isolation from blood sample, Spectrophotometric methods;
Quantification of genomic DNA concentrations using spectrohotometry
Oral presentation;
Laboratory
6. Week
Agarose Gel Electrophoresis of isolated bacterial, plant, mammalian DNA
Oral presentation;
Laboratory
7. Week
Midterm
Agarose gel electrophoresis;
Polyacrylamide gel electrophoresis;
Oral presentation
8. Week
Restriction analysis of genomic DNA
Oral presentation;
Laboratory;
Assignment;
Report
9. Week
Principles of PCR;PCR mechanism;PCR cycle;PCR components; Optimization of PCR;
Application PCR optimization using mammalian genomic DNA gradient PCR applications, Visualization of PCR results with agarose gel electrophoresis
Oral presentation;
Laboratory;
Report
10. Week
Principles of PCR;PCR mechanism;PCR cycle;PCR components; Optimization of PCR;
Application PCR optimization using mammalian genomic DNA gradient PCR applications, Visualization of PCR results with agarose gel electrophoresis
Oral presentation;
Laboratory
11. Week
SNP analysis using RFLP technique and visualization of the RFLP results with polyacrylamide gel electrophoresis
Oral presentation;
Laboratory;
Report
12. Week
Plasmide isolation by Alkali Lysis method
Oral presentation;
Laboratory;
Report
13. Week
Digestion of plasmids by restriction enzymes
Oral presentation;
Laboratory;
Assignment;
Report
14. Week
Purification of PCR products from agarose gel electrophoresis
Oral presentation
15. Week
Final exam
16. Week
Final exam
17. Week
Final exam
Assessments
Evaluation tools
Quantity
Weight(%)
Midterm(s)
1
10
Homework / Term Projects / Presentations
4
40
Attendance / Participation
1
10
Final Exam
1
40
Program Outcomes
PO-1
To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics
PO-2
To be able to identify, model and produce solutions against to problems related to molecular biology and genetics
PO-3
To start a procedure, proceed and finalize about determined problem by using analytical techniques
PO-4
To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics
PO-5
To contribute scientific research and development and add further information
PO-6
To be able to analyze data, design an experiment and proceed and annoate the results
PO-7
To be able to use current techniques and analysis methods in molecular biology and genetics
PO-8
To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes
PO-9
To be able to behave individually, take initiative and creativity skills
PO-10
To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions
PO-11
To carry responsibility to vocational and ethical issues
PO-12
To be aware about quality assessment
PO-13
To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge
Learning Outcomes
LO-1
Ability to understand principles of analyses techniques commonly used in molecular biology.
LO-2
Ability to perform necessary mathematical calculations for laboratory applications.
LO-3
Gaining ability to interpret the results obtained from an experiment based on the techniques used for analyses and optimizing the experimental procedures to get clearer results.
LO-4
Gaining ability to be able to perform purificaiton of macromolecules, gel electrophoresis and spectrophotometric analyses methods.
LO-5
Gaining ability to be able to perform techniques such as PCR and retsriction digestion commonly used for modification of macromolecules.
LO-6
Ability to present the results obtained from a laboratory experiment as a comprehensible report.
LO-7
Ability to select proper experimental procedures, to design procedure steps, to perform and to interprete the results for any molecular biology problem.
