Learning the principles of molecular biology analyses techniques, ability to perform these techniques in guidance with an assistant, ability to interpret experimental results, optimizing the experimental procedures according to the results obtained
Prerequisite(s)
-
Corequisite(s)
-
Special Requisite(s)
-
Instructor(s)
Assist. Prof. Dr. Özge Rencüzoğulları, Assist. Prof. Dr. Burcu Ayhan Şahin, Assist. Prof. Dr. Pelin Özfiliz Kılbaş
Course Assistant(s)
Res. Assist.Pelin Özfiliz, Res. Assist. Burcu Ayhan Şahin, Res. Assist. Dr. Özge Rencüzoğulları, Res. Assist.Tamer Gümüş
Schedule
Tuesday 10:00-14:00 Wednesday 11:00-13:00
Office Hour(s)
Weekdays, Molecular Biology and Genetics Department, M-1-03
Teaching Methods and Techniques
- Power point presentation
- Lecture
- Discussion
- Assistant guided demonstration
- Laboratory application
- Laboratory reports
- Assignments
- Independent study
Principle Sources
1. Editörler Güler Temizkan ve Nazlı Arda, Moleküler Biyolojide Kullanılan Yöntemler, Nobel Tıp Kitapevi, İstanbul, 2007 (ISBN: 9789754205831)
2. Editörler Atilla Özalpan ve Narçin P. Ünsal, Genomik Uygulamalar, T.C. İstanbul Kültür Üniversitesi Yayınları, İstambul, 2008
3. Dale, von Schantz, From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, John Wiley & Sons, Ltd, USA, 2007 (ISBN: 9780470017340)
Other Sources
1. Manuscripts published in scientific journals
Course Schedules
Week
Contents
Learning Methods
1. Week
General information about organic molecules: stock solutions to be prepared for general use in RNA and protein studies
Oral presentation;
Laboratory
2. Week
RNA isolation from plants using Trizol and spectral analysis of isolated RNAs and their demonstration in Agarose Gel Electrophoresis
Oral presentation;
Laboratory
3. Week
RNA isolation using Trizol from mammalian cells and demonstration of isolated RNAs in spectral analysis and Agarose Gel Electrophoresis
Oral presentation;
Laboratory
4. Week
cDNA sythesis, using cDNA as template, RT-PCR and display in gel
Oral presentation;
Laboratory;
Report
5. Week
cDNA production, using cDNA as template, RT-PCR and display in gel
Oral presentation;
Laboratory;
Report
6. Week
Demonstration by qRT-PCR obtained from plant samples
Oral presentation;
Laboratory
7. Week
Demonstration by qRT-PCR obtained from mammalian samples
Oral presentation;
Laboratory;
Report
8. Week
Plant protein isolation, Bradford and Native Gel
Oral presentation;
Laboratory;
Report
9. Week
Mammalian total and nuclear-cytoplasmic protein isolation, Bradford, SDS-PAGE gel preparation
Oral presentation;
Laboratory;
Report
10. Week
SDS-PAGE gel execution, Coomassie staining, transfer, primary-secondary antibody application
Oral presentation;
Laboratory
11. Week
Imaging, strip shooting, Western trouble shooting, IP
Oral presentation;
Laboratory;
Report
12. Week
ELISA, SILAC
Oral presentation;
Laboratory
13. Week
HPLC
Oral presentation;
Laboratory;
Report
14. Week
2-D gel electrophoresis
Student presentations
15. Week
Final exam
16. Week
Final exam
17. Week
Final exam
Assessments
Evaluation tools
Quantity
Weight(%)
Midterm(s)
1
20
Quizzes
1
10
Homework / Term Projects / Presentations
1
10
Final Exam
1
60
Program Outcomes
PO-1
To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics
PO-2
To be able to identify, model and produce solutions against to problems related to molecular biology and genetics
PO-3
To start a procedure, proceed and finalize about determined problem by using analytical techniques
PO-4
To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics
PO-5
To contribute scientific research and development and add further information
PO-6
To be able to analyze data, design an experiment and proceed and annoate the results
PO-7
To be able to use current techniques and analysis methods in molecular biology and genetics
PO-8
To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes
PO-9
To be able to behave individually, take initiative and creativity skills
PO-10
To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions
PO-11
To carry responsibility to vocational and ethical issues
PO-12
To be aware about quality assessment
PO-13
To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge
Learning Outcomes
LO-1
Ability to understand principles of analyses techniques commonly used in molecular biology.
LO-2
Ability to perform necessary mathematical calculations for laboratory applications.
LO-3
Gaining ability to interpret the results obtained from an experiment based on the techniques used for analyses and optimizing the experimental procedures to get clearer results.
LO-4
Gaining ability to be able to perform RNA isolation and analyses methods by without any assistance.
LO-5
Gaining ability to be able to perform protein isolation, gel electrophoresis, quantification and Western blotting techniques without any assistance.
LO-6
Ability to present the results obtained from a laboratory experiment as a comprehensible report.
LO-7
Ability to select proper experimental procedures, to design procedure steps, to perform and to interprete the results for any molecular biology problem.
LO-8
Gaining ability to analyse, comprehend, discuss and present the scientific publicaitons based on experimental approach, interpretation of experimental results and what further experiments required to support the hypothesis presented.
