Course Code	Semester	Course Name	LE/RC/LA	Course Type	Language of Instruction	ECTS
FMG0026		Plant Biotechnology and Applications	1/0/4	DE	Turkish	8

Course Goals	Organogenesis, somatic embryogenesis, protoplast culture and somatic hybridization, haploid plant production, non infected plant production, seconder metabolite production, teoric and application about germplasm production
Prerequisite(s)
Corequisite(s)
Special Requisite(s)
Instructor(s)	Professor Çimen Atak
Course Assistant(s)
Schedule
Office Hour(s)
Teaching Methods and Techniques	Lecturer and student based presentations, laboratory applications
Principle Sources	Evans, D.E,Coleman, J.O.D, Kearns A. Plant Cell Culture, BİOS Scientific Publishers, London and New York , ISBN 1 85996 320 X, 2003. Dicosmo, F,.  Misawa, M, Plant Cell Culture Secondary Metabolism: Towards Industrial Application, CRC-Press; ISBN-13: 978-0849351358, 1996. U. Kumar, Methods in Plant Tissue Culture, Agrobios (India) ISBN13 9788177540772 , 2008. Babaoğlu, B. Gürel, E., Özcan, S. Bitki Biyoteknolojisi S.Ü. vakfı yayınları, ISBN 975-6652-03-9, 2001. Trigiano, R.N., Gray, D. Plant Tissue Culture Concepts and Laboratory Exercise. CRC Pres, ISBN 0-8493-2029-1, 2000. Smith, R.H. Plant Tissue Culture Techniques and Experiments. Academic Pres, 2000. George, E.F., Hall, M.A., Klerk, J.D. Plant Propagation by Tissue Culture 3rd Edition. Volume 1. The Background. Springer ISBN978-1-4020-5004-6.
Other Sources

Course Schedules
Week	Contents	Learning Methods
1. Week	The importance of tissue culture in plant biotechnology, the arrangement of plant tissue culture laboratories, equipments in the laboratories, sterilisation	Teoric ppt presentation
2. Week	Organogenesis and the types, the important issues in organogenesis, establishing of rice cultures	Teoric ppt presentation Laboratory experiment, observations (4-6 weeks)
3. Week	Establishing of tomato tissue cultures	Laboratory experiment, observations (4 weeks)
4. Week	Somatic embryogenesis and somatic embryogenesis culture in carrot	Teoric ppt presentation Laboratory experiment, observations (3-4 weeks)
5. Week	Protoplast culture and somatic hybridization	Teoric ppt presentations Laboratory observations
6. Week	Protoplast isolation and fusions in African violet	Teoric ppt presentation Laboratory experiment, observations (6 weeks) Homework
7. Week	Midterm exam	Exam
8. Week	Haploid plant production and establishing anther culturein African violet	Laboratory experiment, observations (7-8 weeks) Homework
9. Week	Non-disease plant production	Teoric ppt presentation Laboratory observations Homework
10. Week	Non-virus plant productionin Allium sativum	Laboratory experiment Observations (4 weeks)
11. Week	The economic importance of seconder metabolitesi seconder metabolites in medicine, food ingredients, perfumery and agricultural combat	Teoric ppt presentation Laboratory observations Homework
12. Week	Seconder metabolite production	Teoric ppt presentation Laboratory presentations
13. Week	In vitro conservation of plant tissue cultures, decreasing of growth in plant material	Teoric ppt presentation Laboratory observations Homework
14. Week	Conservation of plant material in cold Factors affecting plant vitality	Teoric ppt presentation Laboratory observations Homework
15. Week	Final Exam	Exam
16. Week	Final Exam	Exam
17. Week	Final Exam	Exam

Assessments
Evaluation tools	Quantity	Weight(%)
Midterm(s)	1	25
Homework / Term Projects / Presentations	6	40
Attendance	1	5
Final Exam	1	30

Program Outcomes PO-1 To be able to determine the field related problems and analysis them to find the solutions as a specialist by using the knowledge coming from undergraduate level. PO-2 To be able to develop and apply research strategies, which depend on the new technological achievements, for novel studies about molecular cell biology, plant biotechnology and molecular genetics fields on the basis of undergraduate qualifications. PO-3 To be able to discuss field related subjects by using at least one foreign language. PO-4 To be sensitive against public problems and be able to create solutions to these problems by synthesis of knowledge coming from other disciplines with the abilities and knowledge of the related field PO-5 To be able to take independent decision in the frame of specialized knowledge and scientific approach about field and lead/proceed a project by using creativity skills. PO-6 To be able to decide independently during solving the problem and contribute the group labor in harmony. PO-7 To be able to present information which is supported by quantitative and qualitative data to groups in/outside of the field by written, oral and visually. PO-8 To consider to behave properly in human rights and scientific ethics at research. PO-9 To be able to use informatics and communication skills according required level for area. PO-10 To obtain required quality about field in order to maintain higher academic degree

Learning Outcomes LO-1 Tissue culture applications on different plants LO-2 To betray the importances of tissue cultures on plant biology and economy LO-3 To learn the using of tissue culture techniques in seconder metabolite production which has an important place in industry LO-4 To learn the general principles of protoplast technology, establish of protoplast cultures in laboratories and show the importance of protoplasts in somatic hybridization. LO-5 To establish tissue culture experiments, observations, annotations and discussions of the results

Course Assessment Matrix:

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8	PO 9	PO 10
LO 1
LO 2
LO 3
LO 4
LO 5