In this course, Vectors used E. Coli: Vectors based on E. Coli plasmids, The useful properties of pBR322, The pedgree of pBR 322, Vectors based on M13 bacteriphage, Hybrid plasmid-M13 vectors, Vectors based on l bacteriphage, Insertion and reolacement vectors, Vectors for other bacteria, Vectors for Eukaryotes:, Vectors for yeast and other fungi, vectors based on the 2 µm plasmid-yeast episomal plasmids, Other types of yeast and fungi vectors, Cloning vectors for higher plants:, Ti and Ri plasmidler, Attemps to use plant viruses as cloning vectors, Caulimovirus and Geminivirus, Vectors for animals, Cloning vectors for insects, cloning vectors for mammals subjects are going to be discussed.
Prerequisite(s)
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Corequisite(s)
-
Special Requisite(s)
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Instructor(s)
Professor Özge ÇELİK
Course Assistant(s)
-
Schedule
Not determined yet
Office Hour(s)
Everyday, MOBIGEN building, Atakoy Campus
Teaching Methods and Techniques
-Oral presentation
Principle Sources
-Brown, T. A. (2008) Gene Cloning and DNA analysis an Introduction. Blackwell Science Ltd
Casali, N and Preston, A (2003) E. coli Plasmid Vectors: Methods and Applications. Humana press.
Angel Cid-Arregui, A and Garcia-Carranca, Alejandro (2000) Viral Vectors: Basic Science and Gene Therapy. Eaton Publishing Company/Biotechniques Books.
The definition of vectors which are used in genetics
Lecture
2. Week
Cloning vectors for E.coli
Lecture
3. Week
E-coli plasmid based cloning techniques
Lecture
4. Week
M13 bacteriophage based cloning
Lecture
5. Week
Lambda bacteriophage based cloning
Lecture
6. Week
Utilization of cosmids as cloning vector
Lecture
7. Week
Vectors for other bacteria species
Lecture
8. Week
Midterm
9. Week
Vectors that are used in yeast and fungi
Lecture
10. Week
2um plasmid based vectors
Lecture
11. Week
Yeast episomal vectors
Lecture
12. Week
Cloning vectors for higher plant species: Ti and Ri plasmids
Lecture
13. Week
Caulimovirus and Geminivirus vectors
Lecture
14. Week
Insect and mammalian vectors
Lecture
15. Week
Final exams week
16. Week
Final exams week
17. Week
Final exams week
Assessments
Evaluation tools
Quantity
Weight(%)
Midterm(s)
1
40
Final Exam
1
60
Program Outcomes
PO-1
To be able to determine the field related problems and analysis them to find the solutions as a specialist by using the knowledge coming from undergraduate level.
PO-2
To be able to develop and apply research strategies, which depend on the new technological achievements, for novel studies about molecular cell biology, plant biotechnology and molecular genetics fields on the basis of undergraduate qualifications.
PO-3
To be able to discuss field related subjects by using at least one foreign language.
PO-4
To be sensitive against public problems and be able to create solutions to these problems by synthesis of knowledge coming from other disciplines with the abilities and knowledge of the related field
PO-5
To be able to take independent decision in the frame of specialized knowledge and scientific approach about field and lead/proceed a project by using creativity skills.
PO-6
To be able to decide independently during solving the problem and contribute the group labor in harmony.
PO-7
To be able to present information which is supported by quantitative and qualitative data to groups in/outside of the field by written, oral and visually.
PO-8
To consider to behave properly in human rights and scientific ethics at research.
PO-9
To be able to use informatics and communication skills according required level for area.
PO-10
To obtain required quality about field in order to maintain higher academic degree
Learning Outcomes
LO-1
To know viral, bacterial and yeast vectors
LO-2
To be able to define vectors and their application field
