The term of plant tissue culture will be explain in the base of production of plant and plant products from plant cells and tissues in artificial mediums. While the plant tissue culture techniques are teaching by using laboratory applications, the importance of these techniques on the improvement of plant features, aseptic plant productions, micropropagations, synthetic seed production will be discussed.
Prerequisite(s)
-
Corequisite(s)
-
Special Requisite(s)
-
Instructor(s)
Professor Çimen Atak
Course Assistant(s)
Schedule
Not defined
Office Hour(s)
Every day, Ataköy Campus, MOBIGEN
Teaching Methods and Techniques
Lecturer based presentations, student based presentations, laboratory applications
Principle Sources
Babaoğlu, B. Gürel, E., Özcan, S. Bitki Biyoteknolojisi S.Ü. vakfı yayınları, ISBN 975-6652-03-9, 2001.
Trigiano, R.N., Gray, D. Plant Tissue Culture Concepts and Laboratory Exercise. CRC Pres, ISBN 0-8493-2029-1, 2000.
Smith, R.H. Plant Tissue Culture Techniques and Experiments. Academic Pres, 2000.
George, E.F., Hall, M.A., Klerk, J.D. Plant Propagation by Tissue Culture 3rd Edition. Volume 1. The Background. Springer ISBN978-1-4020-5004-6.
Other Sources
-
Course Schedules
Week
Contents
Learning Methods
1. Week
The historical development of plant tissue culture, the system of plant tissue culture laboratories, sterilization techniques
Dry heat and autoclave sterilization applications
Oral Presentation
Laboratory
2. Week
Mediums, plant growth regulators, plant tissue culture types
The comparison of different sterilization methods on cauliflower tissue cultures
Oral Presentation
Laboratory
3. Week
Callus and cell cultures
Monitoring of the experiments
Oral Presentation
Laboratory
4. Week
Organogenesis technique, media, organogenesis types
Seedling production in tissue culture: Seedlin production in cauliflower plant tissue culture
Oral Presentation
Laboratory
5. Week
Micropropagation,Somatic embryogenesis
The show of micropropagation and plant regeneration: Micropropagation in African violet
Oral Presentation
Laboratory
6. Week
Synthetic seed, gene transfer, Monitoring of the experiments
Monitoring of the experiments
Oral Presentation
Laboratory
7. Week
Midterm Exam
Exam
8. Week
Haploid plant production, Anther culture
Aseptic seed germination: Germination of soybean seeds in aseptic conditions
Oral presentation
Laboratory
9. Week
Microspor culture
Direct organogenesis and callus cultures: Direct organogenesis and callus production is soybean explants
Oral presentation
Laboratory
10. Week
Ovule and ovarium culture
Monitoring of the experiments
Oral presentation
Laboratory
11. Week
Embryo culture
Monitoring of the experiments
Oral presentation
Laboratory
12. Week
Mutations
Monitoring of chlorophyll mutants in M2 generations
Oral prsentation
Laboratory
13. Week
Somaclonal variations
Monitoring of the experiments
Oral presentation
Laboratory
14. Week
Protoplast culture
Monitoring of the experiments
Oral presentation
Laboratory
15. Week
16. Week
17. Week
Assessments
Evaluation tools
Quantity
Weight(%)
Midterm(s)
1
20
Homework / Term Projects / Presentations
5
30
Final Exam
1
50
Program Outcomes
PO-1
To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics
PO-2
To be able to identify, model and produce solutions against to problems related to molecular biology and genetics
PO-3
To start a procedure, proceed and finalize about determined problem by using analytical techniques
PO-4
To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics
PO-5
To contribute scientific research and development and add further information
PO-6
To be able to analyze data, design an experiment and proceed and annoate the results
PO-7
To be able to use current techniques and analysis methods in molecular biology and genetics
PO-8
To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes
PO-9
To be able to behave individually, take initiative and creativity skills
PO-10
To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions
PO-11
To carry responsibility to vocational and ethical issues
PO-12
To be aware about quality assessment
PO-13
To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge
Learning Outcomes
LO-1
Learning of the use of plant tissue culture techniques in plant biotechnology
LO-2
The importance of plant tissue culture in plant breeding applications and other scientific areas
LO-3
Somaclonal variations in plant tissue cultures and the importance of its role in improving new varieties by selection.
LO-4
Using laboratory applications to show the importance of micropropagation
LO-5
Establishment of plant tissue culture experiments,monitoring and commenting their results and writing reports supported by literatures
