Course Code	Semester	Course Name	LE/RC/LA	Course Type	Language of Instruction	ECTS
MBG8004		Animal Cell Culture	1/0/2	DE	Turkish	4

Course Goals	To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader
Prerequisite(s)	-
Corequisite(s)	-
Special Requisite(s)	-
Instructor(s)	Assist. Prof. Dr. Özge Rencüzoğulları
Course Assistant(s)	Res. Assist. Pinar Obakan
Schedule	Not determined
Office Hour(s)	Prof. Dr. Atilla Özalpan, Dean Faculty of Science and Literature, 4300
Teaching Methods and Techniques	-ppt presentations - discussion
Principle Sources	-
Other Sources	-

Course Schedules
Week	Contents	Learning Methods
1. Week	Basic terms and concepts. Tissue culture laboratory, required devices and equipments	Oral presentation, demonstration
2. Week	Aseptic techniques	Oral presentation, demonstration
3. Week	Animal cell culture medias	Oral presentation, demonstration
4. Week	Maintaining of primary cell cultures and cell lines	Oral presentation, demonstration
5. Week	How to use laminal flow, CO2 incubators, organize the cell cultre room	Oral presentation, demonstration
6. Week	General techniques in cell culture: determine the cell types, seeding conditions	Oral presentation, demonstration
7. Week	Midterm	Analysis and synthesis
8. Week	Cell counting methods, cell seeding and culturing	Oral presentation, application
9. Week	Cell preperation, cell freeze, and thawing cells	Oral presentation, application
10. Week	Determine cell viabilty: MTT methods	Oral presentation, application
11. Week	Determine cell viability: Trypan blue staning	Oral presentation, application
12. Week	To determine cell death: PI staining	Oral presentation, application
13. Week	To determine the various metabolic functions in cells: determination of Reactive oxygene species derivatives by DCFDH staining	Oral presentation, application
14. Week	To determine the various metabolic functions in cells: determining mitochondrial potantial by DIOC6 staining	Oral presentation, application
15. Week	Final exam
16. Week	Final exam
17. Week	Final exam

Assessments
Evaluation tools	Quantity	Weight(%)
Midterm(s)	1	30
Homework / Term Projects / Presentations	1	10
Attendance	1	5
Final Exam	1	55

Program Outcomes PO-1 To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics PO-2 To be able to identify, model and produce solutions against to problems related to molecular biology and genetics PO-3 To start a procedure, proceed and finalize about determined problem by using analytical techniques PO-4 To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics PO-5 To contribute scientific research and development and add further information PO-6 To be able to analyze data, design an experiment and proceed and annoate the results PO-7 To be able to use current techniques and analysis methods in molecular biology and genetics PO-8 To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes PO-9 To be able to behave individually, take initiative and creativity skills PO-10 To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions PO-11 To carry responsibility to vocational and ethical issues PO-12 To be aware about quality assessment PO-13 To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge

Learning Outcomes LO-1 To learn how to maintain cell survalival in aseptic and controlable conditions LO-2 To learn and apply the cell culture principles LO-3 To discriminate requirements of the cell culture conditions LO-4 To determine the cell viability by quantitative and qualitative ways LO-5 To determine the cell death by quantitative and qualitative ways LO-6 To define the cellular metabolic fuınctions by the guide of fluorescence microscopy

Course Assessment Matrix:

PO 1

PO 2

PO 3

PO 4

PO 5

PO 6

PO 7

PO 8

PO 9

PO 10

PO 11

PO 12

PO 13

LO 1

LO 2

LO 3

LO 4

LO 5

LO 6