In this course, students are able to define genetic engineering term and receive information about genetics engineering methods that used for production, application field, production of genetic modified organisms
Prerequisite(s)
-
Corequisite(s)
-
Special Requisite(s)
Molecular Biology Techniques I
Instructor(s)
Assist. Prof. Dr. Sinan Meriç
Course Assistant(s)
-
Schedule
Friday; 09:00-11:00
Office Hour(s)
Assoc. Prof. Dr. Ajda Çoker Gürkan, Thursday; 14:00-15:00, Atakoy campus, AK/M-Z-09
Teaching Methods and Techniques
- Powerpoint presentations of courses
-Reading and discussing papers related to course subject.
- Video demonstrations linked to couse subject
Principle Sources
-An Introduction to Genetic Engineering, DST. Nicholl, 3. Edition, Cambridge University Press, 2008.
-Introduction to Biotechnology and Genetic Engineering, A J. Nair, Jones & Bartlett Publishers, 2008.
-Genetic Engineering (Genetics & Evolution), R. Hodge, Facts on File, 2009.
-Principles of Gene Manipulation and Genomics, SB. Primrose, R. Twyman, 7. Edition, Wiley-Blackwell, 2006.
-Gene Cloning and DNA Analysis: An Introduction, T. Brown, 6. Edition, Wiley-Blackwell, 2010.
-Gene Therapy: Treatments and Cures for Genetic Diseases, J. Panno, Facts on File (J), 2010.
Other Sources
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-www.sciencedirect.com
-www.ncb.nlm.nih.gov
Course Schedules
Week
Contents
Learning Methods
1. Week
Introduction to genetic engineering, applications of genetic engineering products and cloning strategies
Cloning vector types, vector systems, host cells and organisms and their using strategies
- Oral presentation, discussion, demonstration
3. Week
Genomic and cDNA cloning steps, strategies and teorical application by using bioinformatic tools
- Oral presentation, discussion, demonstration
4. Week
Generation of Genomic DNA and cDNA libraries and strategies for DNA library
- Oral presentation, discussion, demonstration
5. Week
Site-direct mutagenesis steps, types and strategies
- Oral presentation, discussion, demonstration
6. Week
Site-direct mutagenesis theorical application by using bioinformatic tools
- Oral presentation, discussion, demonstration
7. Week
Midterm exam
- Analysis, synthesis and evaluation
- Oral presentation, discussion, demonstration
8. Week
Recombinant fusion protein cloning, expression and purification steps and theorical applications by using bioinformatic tools
- Oral presentation, discussion, demonstration
9. Week
Genetic reporter systems: GFP, mCherry, Luciferase reporter arrays and their applications in vitro and in vivo
- Analysis, synthesis and evaluation
- Oral presentation, discussion, demonstration
10. Week
siRNA and miRNA applications and strategies
- Oral presentation, discussion, demonstration
11. Week
Actual scientific paper reading and discussion
- Analysis, synthesis and evaluation
- Oral presentation, discussion, demonstration
12. Week
Actual scientific paper reading and discussion
- Analysis, synthesis and evaluation
- Oral presentation, discussion, demonstration
13. Week
Actual scientific paper reading and discussion
- Analysis, synthesis and evaluation
- Oral presentation, discussion, demonstration
14. Week
Actual scientific paper reading and discussion
- Oral presentation, discussion, demonstration
15. Week
Final exam week: Oral presentaiton
-Analysis, synthesis and evaluation
16. Week
Final exam week: final exam
-Analysis, synthesis and evaluation
17. Week
Final exam week:
-Analysis, synthesis and evaluation
Assessments
Evaluation tools
Quantity
Weight(%)
Midterm(s)
1
40
Homework / Term Projects / Presentations
1
0
Attendance
1
10
Final Exam
1
50
Program Outcomes
PO-1
To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics
PO-2
To be able to identify, model and produce solutions against to problems related to molecular biology and genetics
PO-3
To start a procedure, proceed and finalize about determined problem by using analytical techniques
PO-4
To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics
PO-5
To contribute scientific research and development and add further information
PO-6
To be able to analyze data, design an experiment and proceed and annoate the results
PO-7
To be able to use current techniques and analysis methods in molecular biology and genetics
PO-8
To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes
PO-9
To be able to behave individually, take initiative and creativity skills
PO-10
To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions
PO-11
To carry responsibility to vocational and ethical issues
PO-12
To be aware about quality assessment
PO-13
To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge
Learning Outcomes
LO-1
To define genetic engineering term and recognize molecular biology and genetic methods for applications of genetic engineering
LO-2
To distinguish genetic modified organisms and cloning strategies
LO-3
To be able make a scheme about protein, enzyme or antibody production by genetic engineering approach and purification steps
LO-4
To be able to discuss the applications of genetic engineering in agriculture, veterinary, medicine, health and criminal sciences
LO-5
To be able to design a scientific project by using the fundamental methods and principles of genetic engineering
LO-6
To be able to discuss current literature about genetic engineering products or genetic modifed organisms
