Learning of plant transformation techniques, molecular biology techniques used in analysis of genetically modified plants and laboratory studies of these analysis.
Prerequisite(s)
-
Corequisite(s)
-
Special Requisite(s)
-
Instructor(s)
Assist. Prof. Dr. Sinan Meriç
Course Assistant(s)
Res.Assist. Alp Ayan
Schedule
Not defined
Office Hour(s)
Every day, Ataköy Campus, MOBIGEN
Teaching Methods and Techniques
Lecturer and student based presentation
Principle Sources
1.Plant Biotechnology and Genetics, Principles, Techniques and Applications, C. Neal Stewart JR. (ED), Wiley, 2008, ISBN: 978-0-470-04381-3
2.Plant Biotechnology- The genetic manipulation of plants, Adrian Slater, Nigel, Scott, Mark Fowler, Oxford University Press, 2004, ISBN: 019925468
3.Plant Biotechnology and Development, Peter M. Gresshoff (Ed.), Current Topics in Plant Molecular Biology
Other Sources
Course Schedules
Week
Contents
Learning Methods
1. Week
Structure of the gene, gene expression, regulation of gene expression in plants
Working in plant biotechnology laboratories
Oral presentation
Laboratory
2. Week
Introduction to plant transformation methods
Solution and medium preparation
Oral presentation
Laboratory
3. Week
Agrobacterium tumefaciens, Ti plasmid
A. tumefaciens culture conditions, culture growth
Oral presentation
Laboratory
4. Week
Transformation via A. tumefaciens, Quiz
Transformation of Tobacco plant with A. tumefaciens
Oral presentation
Laboratory
5. Week
Direct gene transfer methods
Transformation of Tobacco plant with A. tumefaciens
Oral presentation
Laboratory
6. Week
Pasmid vectors and their features
Tobacco gene transfer application-transgenic plant growth
Oral presentation
Laboratory
7. Week
Midterm Exam
Exam
8. Week
Plant transformation vectors and their features
Tobacco gene transfer application-transgenic plant analysis
Oral presentation
Laboratory
9. Week
Marker and reporter genes used in plant transformation vectors
Tobacco gene transfer application-transgenic plant analysis
Biosafety in plant biotechnology applications
Lab project presentations
Oral presentation
Laboratory
15. Week
16. Week
17. Week
Assessments
Evaluation tools
Quantity
Weight(%)
Midterm(s)
1
30
Quizzes
2
0
Homework / Term Projects / Presentations
1
20
Final Exam
1
50
Program Outcomes
PO-1
To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics
PO-2
To be able to identify, model and produce solutions against to problems related to molecular biology and genetics
PO-3
To start a procedure, proceed and finalize about determined problem by using analytical techniques
PO-4
To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics
PO-5
To contribute scientific research and development and add further information
PO-6
To be able to analyze data, design an experiment and proceed and annoate the results
PO-7
To be able to use current techniques and analysis methods in molecular biology and genetics
PO-8
To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes
PO-9
To be able to behave individually, take initiative and creativity skills
PO-10
To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions
PO-11
To carry responsibility to vocational and ethical issues
PO-12
To be aware about quality assessment
PO-13
To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge
Learning Outcomes
LO-1
The definition of transgenic plant and having information about the production methods
LO-2
Learning of plant transformation techniques
LO-3
The investigation of plant transformation vectors
LO-4
Having knowledge on transgenic plant analysis
LO-5
Discussing of genetically modified plants and biosafety
