Course Code	Semester	Course Name	LE/RC/LA	Course Type	Language of Instruction	ECTS
MBG7013		Transgenic Animal Models	2/0/0	DE	Turkish	4

Course Goals	The aim of the course is understanding the management, organisation of animal laboratory, discriminate the laboratory animal model type, transgenic animal production protocols, genome editing of transgenic animals by using Zinc Finger Nuclease, TALEN, CRISPR/Cas9 methods.
Prerequisite(s)	-
Corequisite(s)	-
Special Requisite(s)	-
Instructor(s)	Assist. Prof. Dr. Özge Rencüzoğulları
Course Assistant(s)
Schedule	Tuesday; 15:00-17:00, CATS online
Office Hour(s)	Tuesday 14:00-15:00 cats chat room
Teaching Methods and Techniques	-Lecture power point demonstration - Video presentation - Scientific paper readig
Principle Sources	-Guide for the care and the use of laboratory animals, National Academies, Eigth Edition Clinical Laboratory Animal Medicine, An Introduction, Karen Hrapkiewicz, Lesley Colby, Patricia Denision - Transgenic Animals Generation and Use, Louis Marie Houdebine
Other Sources	-www.ncbi.nlm.nih.gov - www.sciencedirect.com

Course Schedules
Week	Contents	Learning Methods
1. Week	Introduction of Transgenic Animal Models	Oral presentation, demonstration
2. Week	The organisation and management of laboratory animal conditions.	Oral presentation, demonstration
3. Week	Gene Transfer: Invertebrate and fish	Oral presentation, demonstration
4. Week	Gene Transfer: Bird Embryo	Oral presentation, demonstration
5. Week	Gene Transfer: Mammalian Embryo	Oral presentation, demonstration
6. Week	Genome Editing: Zinc Finger Nuclease technology	Oral presentation, demonstration
7. Week	Midterm Exam
8. Week	Genome Editing TALEN technology	Oral presentation, demonstration
9. Week	Genome Editing CRISPR/Cas9 technology	Oral presentation, demonstration
10. Week	Scientific papers about C.elegans Genome Editing	Oral presentation, demonstration
11. Week	Scientific papers about Drosophila Genome Editing	Oral presentation, demonstration
12. Week	Scientific papers about Danio rerio Genome Editing	Oral presentation, demonstration
13. Week	Scientific paper about Mice/Rats Genome Editing	Oral presentation, demonstration
14. Week	Scientific paper about Monkey/Pig Genome Editing	Oral presentation, demonstration
15. Week	Project demonstration	Oral presentation, demonstration
16. Week	Project demonstration	Oral presentation, demonstration
17. Week	Final Exam

Assessments
Evaluation tools	Quantity	Weight(%)
Midterm(s)	1	10
Homework / Term Projects / Presentations	2	40
Attendance	1	10
Final Exam	1	40

Program Outcomes PO-1 To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics PO-2 To be able to identify, model and produce solutions against to problems related to molecular biology and genetics PO-3 To start a procedure, proceed and finalize about determined problem by using analytical techniques PO-4 To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics PO-5 To contribute scientific research and development and add further information PO-6 To be able to analyze data, design an experiment and proceed and annoate the results PO-7 To be able to use current techniques and analysis methods in molecular biology and genetics PO-8 To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes PO-9 To be able to behave individually, take initiative and creativity skills PO-10 To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions PO-11 To carry responsibility to vocational and ethical issues PO-12 To be aware about quality assessment PO-13 To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge

Learning Outcomes

Course Assessment Matrix:

PO 1

PO 2

PO 3

PO 4

PO 5

PO 6

PO 7

PO 8

PO 9

PO 10

PO 11

PO 12

PO 13