Learning the principles and applicaiton of commonly used techniques in recombinant DNA technology, learning the criteria for the vector selection, understanding the principles and applications for advanced techniques used in recombinant DNA technology, preparing a goal-oriented project, selection of experimental approaches, their laboratory applicaiton, interprtation of results and preparing a final report presenting and discussing the findings
Prerequisite(s)
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Corequisite(s)
-
Special Requisite(s)
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Instructor(s)
Assist. Prof. Dr. Sinan Meriç
Course Assistant(s)
Res. Asist. Sinan Meriç
Schedule
Monday 13.00-16.00
100% Distance learning via CATS platform
Office Hour(s)
Ataköy Campus
Online meeting via CATS platform
Teaching Methods and Techniques
- Power point presentation
- Lecture
- Discussion
- Independent study
- Animation and videos
- Internet search
- Laboratory
Principle Sources
1. Gene Cloning and DNA Analysis: An Introduction, 6th Edition, T. Brown, Wiley-Blackwell, ISBN: 978-1-4051-8173-0, 2010
2. From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, Dale, von Schantz, John Wiley & Sons, Ltd, ISBN: 978-0-470-01734-0, 2007
3. Principles of Gene Manipulation and Genomics, 7th Edition, Primrose, Twyman, Blackwell Publishing, ISBN: 978-1-4051-3544-3, 2006
Other Sources
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Course Schedules
Week
Contents
Learning Methods
1. Week
Introduction to recombinant DNA technology;
Selection of the organism and the gene to be cloned in the project
Oral presentation;
Project
2. Week
Basic techniques in recombinant DNA technology;
Obtaining targeted DNA molecule;
Ligation of targeted DNA molecule into cloning vehicles;
Transformation of chimeric DNA to host cells;
Selection of host cells carrying the chimeric DNA molecule and methods used in plasmid selection;
Genomic DNA isolation
Oral presentation;
Project
3. Week
Vectors;
Plasmids;
Bacteriophages;
Cosmids;
M13 vectors (Single stranded vectors);
Confirmation of genomic DNA isolation
Oral presentation;
Project
4. Week
Gene expression vectors;
Yeast vectors;
Mammalian cell vectors;
Super vectors (BAC, YAC, MAC, HAC);
Primer design for PCR amplification of targeted gene
Oral presentation;
Project
5. Week
Gene transfer methods;
Chemical transformation;
Electroporation;
Gene transfer to animals;
Gene transfer to plants;
PCR amplification
Oral presentation;
Project
6. Week
Gene cloning strategies;
Cloning whole genome/transcriptome;
Visualization of PCR amplification results by agarose gel electrophoresis;
Purification of amplicon from agarose gel
Oral presentation;
Project
7. Week
Specific cloning;
Verification of gel extraction
Oral presentation;
Project
8. Week
Cloning and screening of genomic and cDNA libraries;
Random genome fragmentation;
Partial digestion of genome;
Vector selection for genomic libraries;
Cloning of genomic libraries and evaluation of results;
Storage of genomic libraries;
Selection of vector system and proper restriction enzymes for cloning
Oral presentation;
Project
9. Week
Cloning of cDNA libraries;
Random, structured and ordered libraries;
Screening of libraries with gene probes;
Subcloning;
Vector cloning
Oral presentation;
Project
10. Week
Analyses of gene expression and function;
Analyses of transcription;
Analyses of transcriptome;
Promotor activity analyses;
Analyses of translation;
Verification of cloning with restriction digestion
Oral presentation;
Project
11. Week
Genome mapping;
Transposon mutation libraries;
Gene knockout;
Complementation analyses;
Analyses of gene function from protein-protein interactions;
Transformation of chimeric vector to bacteria and agar selection
Oral presentation;
Project
12. Week
Production of recombinant protein;
Production of recombinant protein in bacteria;
Protein modifications;
Plasmid isolation from bacteria
Oral presentation;
Project
13. Week
Transgenic approaches;
Verification of cloned gene in plasmids using restriction digestion
Oral presentation;
Project
14. Week
Presentation of project results
Student presentation
15. Week
Final exam
16. Week
Final exam
17. Week
Final exam
Assessments
Evaluation tools
Quantity
Weight(%)
Midterm(s)
1
10
Homework / Term Projects / Presentations
1
20
Project(s)
1
20
Attendance
1
10
Final Exam
1
40
Program Outcomes
PO-1
To apply all knowledge about basic sciences such as mathematics, physics, chemistry and biology to all problems in molecular biology and genetics
PO-2
To be able to identify, model and produce solutions against to problems related to molecular biology and genetics
PO-3
To start a procedure, proceed and finalize about determined problem by using analytical techniques
PO-4
To be able to understand all knowledge about living organisms which is main subject of molecular biology and genetics
PO-5
To contribute scientific research and development and add further information
PO-6
To be able to analyze data, design an experiment and proceed and annoate the results
PO-7
To be able to use current techniques and analysis methods in molecular biology and genetics
PO-8
To be able to be a part of a team in disciplinary or multidisciplinary, national or international public problems focused projects and use at least one foreign language for vocational purposes
PO-9
To be able to behave individually, take initiative and creativity skills
PO-10
To communicate briefly and clear using oral or written presentation skills to present individual working performance and independent decisions
PO-11
To carry responsibility to vocational and ethical issues
PO-12
To be aware about quality assessment
PO-13
To adopt in principle the importance of life-learning subject and graduate self-development by following new literature about field and apply current knowledge
Learning Outcomes
LO-1
Understanding the usage of common molecular biology techniques in recombinant DNA technology.
LO-2
Learning the principles of advanced techniques in recombinant DNA technology and interpretation of results obtained by these techniques.
LO-3
Gaining ability to prepare a goal-oriented project, to select proper laboratory experiments, to perform experiments and to interpret the results obtained.
LO-4
Gaining ability to perform a project using the basic recombinant DNA technology techniques by him/herself.
LO-5
To write a final report of the project and to make a presentation.
LO-6
Understanding how to create a transgenic animal or plant and their area of usage and gaining ability to discuss in ethical perspective.
